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Image Search Results


Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Journal: Journal of Pain Research

Article Title: Effects and Mechanisms of Wrist-Ankle Acupuncture on Inflammatory Pain in the Rat Gastrocnemius Muscle

doi: 10.2147/JPR.S605568

Figure Lengend Snippet: Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Article Snippet: Complete freund’s adjuvant (CFA) (Sigma, USA, F5881), trichloroethanol (Aibei, Nanjing, CN, M2820), acupuncture needles (Jiajian Medical, CN), TENS-WAA equipment (Shanghai MicroPort Scientific Co, CN), Von Frey hairs mechanical stimulation needles (Yuyan Instrument, CN), Von Frey test cage (Yuyan Instrument, CN), Rat IL-6 ELISA Kit (Servicebio, CN, GER0001-96T), Rat IL-1β ELISA Kit (Servicebio, CN, GER0002-96T), Rat TNF-α ELISA Kit (Servicebio, CN, GER0004-96T), Rat TGF-β1 ELISA Kit (Servicebio, CN, GER0051-96T), BCA Protein Assay Kit (Servicebio, CN, G2026-1000T), TRIzol ® reagent (China, Servicebio, CN, G3013) SweScript All-in-One SuperMix for qPCR (Servicebio, CN, G3337), Blue SYBR Green qPCR Master Mix (Servicebio, CN, G3326).

Techniques:

Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Journal: Journal of Pain Research

Article Title: Effects and Mechanisms of Wrist-Ankle Acupuncture on Inflammatory Pain in the Rat Gastrocnemius Muscle

doi: 10.2147/JPR.S605568

Figure Lengend Snippet: Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Article Snippet: Complete freund’s adjuvant (CFA) (Sigma, USA, F5881), trichloroethanol (Aibei, Nanjing, CN, M2820), acupuncture needles (Jiajian Medical, CN), TENS-WAA equipment (Shanghai MicroPort Scientific Co, CN), Von Frey hairs mechanical stimulation needles (Yuyan Instrument, CN), Von Frey test cage (Yuyan Instrument, CN), Rat IL-6 ELISA Kit (Servicebio, CN, GER0001-96T), Rat IL-1β ELISA Kit (Servicebio, CN, GER0002-96T), Rat TNF-α ELISA Kit (Servicebio, CN, GER0004-96T), Rat TGF-β1 ELISA Kit (Servicebio, CN, GER0051-96T), BCA Protein Assay Kit (Servicebio, CN, G2026-1000T), TRIzol ® reagent (China, Servicebio, CN, G3013) SweScript All-in-One SuperMix for qPCR (Servicebio, CN, G3337), Blue SYBR Green qPCR Master Mix (Servicebio, CN, G3326).

Techniques:

Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Journal: Journal of Pain Research

Article Title: Effects and Mechanisms of Wrist-Ankle Acupuncture on Inflammatory Pain in the Rat Gastrocnemius Muscle

doi: 10.2147/JPR.S605568

Figure Lengend Snippet: Inflammatory cytokine levels in the gastrocnemius muscle of rats in each group. ( a ) IL-1β; ( b ) IL-6; ( c ) TNF-α; ( d ) TGF-β (n = 4). Data are presented as mean ± SEM. Differences between groups were compared using one-way ANOVA. * p < 0.05, ns p > 0.05.

Article Snippet: Complete freund’s adjuvant (CFA) (Sigma, USA, F5881), trichloroethanol (Aibei, Nanjing, CN, M2820), acupuncture needles (Jiajian Medical, CN), TENS-WAA equipment (Shanghai MicroPort Scientific Co, CN), Von Frey hairs mechanical stimulation needles (Yuyan Instrument, CN), Von Frey test cage (Yuyan Instrument, CN), Rat IL-6 ELISA Kit (Servicebio, CN, GER0001-96T), Rat IL-1β ELISA Kit (Servicebio, CN, GER0002-96T), Rat TNF-α ELISA Kit (Servicebio, CN, GER0004-96T), Rat TGF-β1 ELISA Kit (Servicebio, CN, GER0051-96T), BCA Protein Assay Kit (Servicebio, CN, G2026-1000T), TRIzol ® reagent (China, Servicebio, CN, G3013) SweScript All-in-One SuperMix for qPCR (Servicebio, CN, G3337), Blue SYBR Green qPCR Master Mix (Servicebio, CN, G3326).

Techniques: